In 1999, Eskildsen graduated as design manager from the design academy in Kolding. After finishing her degree she started working for IC Companys (a Danish listed fashion group). In 2002 she established Designers Remix by Charlotte Eskildsen with her husband. At the beginning the brand was owned by IC Companys. However, in 2009 the couple acquired 49% of the stock of Designers Remix A/S.

As creative director for Designers Remix she had a strong design signature. She took an architectural approach to the design process. Her approach employs artistic ideas, minimalism, 3D tailoring, draping and cutting. Her vision is to create an avant-garde angle on modern classics such as the tuxedo and the little black dress. Her inspiration springs from her Danish heritage. Danish design icons, organic modernism and functionalism appear throughout creations.

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